Rationalizing Directed Evolution through Protein Dynamics
نویسندگان
چکیده
منابع مشابه
Laboratory-directed protein evolution.
Systematic approaches to directed evolution of proteins have been documented since the 1970s. The ability to recruit new protein functions arises from the considerable substrate ambiguity of many proteins. The substrate ambiguity of a protein can be interpreted as the evolutionary potential that allows a protein to acquire new specificities through mutation or to regain function via mutations t...
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Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or synergistic mutations are required for the creation of a novel protein function. Here we review the key developm...
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* 1 CUBIC, Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168 Street BB217, New York, NY 10032, USA; 2 Columbia University Center for Computational Biology and Bioinformatics (C2B2), Russ Berrie Pavilion, 1150 St. Nicholas Avenue, New York, NY 10032, USA; 3 North East Structural Genomics Consortium (NESG), Department of Biochemistry and Molecular Biophysics, ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.3619